GHK-Cu is the copper-chelated tripeptide Gly-His-Lys-Cu(II) that first showed up in Loren Pickart's 1973 work on wound healing and has since become the single most-studied peptide in cosmetic science
*Correspondence: Fang Deng, [email protected] This article was submitted to Inflammation, a section of the journal Frontiers in Immunology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
Instead, try to eat several smaller meals throughout the day
Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
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