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The supernatant was mixed with 2 M perchloric acid (10 l supernatant in 100 l perchloric acid)

But the primary concern is the risk of ochronosis, a rare but challenging condition where long-term overuse paradoxically causes darkening of the skin, says Francesa Lewis, MD, a board-certified dermatologist in Delray Beach, Florida

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All metabolites were normalized with total analyzed cell numbers for analysis