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In the samples treated with 0.1, 0.3, and 0.5 mM of tBuOOH, the Gaussian distribution of relative velocities retained values X c = 0.311 a.u

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Nested PCR and linked product amplification To generate sufficient linked amplicon product for library generation while retaining library diversity, PCR product were subjected to less than 15 cycles of nested PCR amplification

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