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[] Serum levels of total testosterone, free testosterone, total estrogen, estradiol, PSA, and total cholesterol were measured at baseline, mid-point, and before radical prostatectomy

Expanded NK cells were cultured in RPMI (Corning) supplemented with 10% FBS (Genesee Scientific) + 1% of each of the following: penicillin/streptomycin (HyClone), NEAA (Lonza), L-glutamine (Sigma), sodium pyruvate (Lonza), and HEPES (ThermoFisher)

In the GHK-Cu complex, the copper ion coordinates with the nitrogen atom from the imidazole side chain of histidine, the alpha-amino group nitrogen from glycine, and the deprotonated amide nitrogen of the glycine-histidine peptide bond