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Polyhistidine tags containing less than six histidines typically require less imidazole for elution, while polyhistidine proteins containing more than six polyhistidines may require higher levels of imidazole

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M., Schnackenberg, J., Raote, S., Srivastava, S., Negi, P., & Caston, E

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To determine if an environment is safe, practitioners often recommend specialized environmental testing, such as the Environmental Relative Moldiness Index (ERMI) or the HERTSMI-2 test