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It is also known by the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val

GPX4 specific activity was carried out in a coupled assay, by monitoring the consumption of NADPH in the presence of glutathione reductase upon addition of phosphatidyl choline hydroperoxide ( Viability Assay Cells were seeded onto 96-well plates (5,000 cells per well) and allowed to attach overnight

This arrangement presumably prevents the electrostatic repulsion of the two glutathione moieties on the surface of active glutaredoxins

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[DOI] [PMC free article] [PubMed] [Google Scholar] 142.Zhang H., Davies K.J., Forman H.J