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Western blot Total proteins from ovarian tissues and cells were extracted using radioimmunoprecipitation assay lysis buffer (Beyotime) and quantified by bicinchoninic acid method

M1 macrophages are characterized by high inducible nitric oxide synthase (iNOS) expression and the secretion of pro-inflammatory factors, whereas M2 macrophages express molecules such as arginase-1 (Arg1) and interleukin-10 (IL-10) and participate in tissue repair ( in vivo do not exist as fixed binary states, but rather display a continuum of functions shaped jointly by microenvironmental signals, metabolic reprogramming, and epigenetic regulation ( In recent years, studies of immunometabolism have gradually revealed the important role of metabolic reprogramming in regulating immune cell function

^up to 6 weeks from diagnosis

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Eating before drinking makes more of a difference than it used to