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Description

Samples were then washed 2 times with 1 mL staining buffer before being run through BD FACSCanto II flow cytometry (BD Bioscience, Franklin Lakes, New Jersey, USA)

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The resultant files were processed with El-Maven (Elucidata) alongside the Kyoto Encyclopedia of Genes and Genomes (KEGG) 20,21,22 database for metabolite assignment and manual peak integration as previously described using intact mass, isotopic patterns, and an in-house standard library 23

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This procedure was performed for 30 min when participants were fasting

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