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1S10OD025120 for the 10X Chromium housed in the NUSeq Facility, and NIH grant no

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Methods Construct cloning The full-length human ghrelin receptor (residues 1366) was sub-cloned into pFastBac1 vector with an N-terminal haemagglutinin signal peptide (HA) and His10 tag followed by BRIL epitope, as well as LgBiT at the C-terminus to facilitate the protein expression and stability

You need proper technique, sterile supplies, and careful attention to protocol