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The supernatant was added to beads (equilibrated with 200 mM NaCl, 50 mM TrisHCl, pH 7.5, 10% glycerol, 5 mM choline chloride, 0.05% LMNG) in a 10-ml gravity column at 4C and sequentially washed with 2 1 ml of buffer containing 0.01% LMNG

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J Clin Invest 121:20942101