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Methods Cell culture, expression constructs, and transfection MIN-6 (purchased from ATCC) cells and GLUTag cells (obtained from Takashi Tsuboi, the university of Tokyo, Japan) were cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum

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Acetonitrile (125 ml) was added to the obtained compound at 25-30C

The processes leading to increased arterial stiffness are related to structural remodelling such as increased collagen and decreased elastin in the vessel wall, as well as higher resting vascular tone, endothelial dysfunction, and reduced nitric oxide bioavailability (Lyle and Raaz, 2017)

GLP-1 medications were initially developed to treat diabetes but have recently gained widespread recognition for their significant effectiveness in promoting weight loss