Cells were pelleted at 300 g for 6 min, resuspended in complete MEM with 50 g ml 1 Gen at 2.5 10 5 cells per ml, and dispensed (80 l per well) into 384-well white and black plates (ThermoFisher) for luminescence and fluorescence measurements, respectively
The paw withdrawal latency (PWL) to heat was measured after either intraperitoneal or oral gavage administration of glucose (2.0 g/kg body weight) in a volume of 200 L or the same volume of vehicle (0.9% saline) ( n = 5 per group)
Chen N, Appell M, Berfield JL, Reith ME
Im going to order my refill to start on my second month
van Wijngaarden E, Davidson PW, Smith T, Evans K, Yost K, Love T, Thurston SW, Watson GE, Zarba G, Burns C, Shamlaye CF, Myers GJ (2013) Autism spectrum disorder phenotypes and prenatal exposure to methylmercury