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The full-length PGRMC1 cDNA fragment containing resistant sequences for shRNA was generated by using the primers (Supplementary Methods), and ligated into the Eco RI and Bam HI sites of the C-terminus of the 3xFLAG-tagged expression vector p3xFLAG CMV14 (Sigma)

Berk M, Dean O, Cotton SM, Gama CS, Kapczinski F, Fernandes BS, Kohlmann K, Jeavons S, Hewitt K, Allwang C, Cobb H, Bush AI, Schapkaitz I, Dodd S, Malhi GS

Piperlongumine and its analogs down-regulate expression of c-Met in renal cell carcinoma

Neurology 96 , e2098e2108 (2021)

Mitochondrial dysfunction plays a key role in the development of neurodegenerative diseases in diabetes