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Nanofcm, Fujian, China) was utilized to analyze particle size distribution and concentration of EV samples following the manufacturers manual and as referenced in a previous report 22

However, ASP levels showed a nonsignificant change in the thalamus and hypothalamus ( F (5, 24) = 2.217, p = 0.086, W = 0.736, p = 0.604) in all experimental groups, while there was a significant increase after 15 days of intermittent fasting (group F3) compared to group F2 ( p = 0.05)

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