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The effect was initially thought to be secondary to reduced gastric emptying, however, it was later shown to be an independent effect (227, 228)

In brief, HES2 cells were dissociated with Accutase (Invitrogen) into single-cell suspensions on day 0

Both in vitro and in vivo models, as well as clinical and epidemiological data have indicated the role of the IGF-1 system in BrCa via many diverse endocrine, paracrine and autocrine signaling pathways [16,17]

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Failure to diagnose and treat in time, however, may result in permanent neurological damage, excessive fatigue, depression, memory loss, and other complications