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For the purposes of comparison between studies, the precise location of the region studied was recorded, or if not available, information on primer design was collected to retrieve genomic coordinates via in silico PCR [21]

Immune-related markers can provide information about changes in the tumor microenvironment, which can elucidate the immune status of BC, as well as elucidate the mechanism of different immune cells in the tumor microenvironment ( 2 Immune-related markers in the diagnosis of bladder cancer 2.1 Immune-related genes 2.1.1 FGFR3 FGFR3 gene mutations have been found to be highest in BC and are associated with cancer cell proliferation and migration, and tumor invasiveness ( 2.1.2 MAN1B1 The study demonstrated higher MAN1B1 expression in bladder cancer patients than in normal tissues from The Cancer Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases

0.5 TrypLE Select solution was prepared with 50% TrypLE Select, no phenol red (Thermo Fisher Scientific) and 0.25 mM EDTA (0.5 mol/L EDTA Solution (pH 8.0), Nacalai Tesque Inc.) in DPBS () for single-cell culture

It offers a different approach to managing diabetes and weight loss

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