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Materials and methods Generation of Braf CA/wt ,Mieap KO/KO and Braf CA/wt ,Atg5 flox/flox mice Braf CA (B6.129P2(Cg) Braf tm1Mmcm /J, stock# 017837) mice ( Braf V600E , were obtained from Jackson Laboratory (Bar Harbor, ME, USA)

Because these agents inhibit transmission in both sympathetic and parasympathetic ganglia, physiological effects reflect blockade of the predominant resting autonomic tone, including tachycardia (cardiac) and marked orthostatic hypotension (vascular) (middle panel)

the impact on the development of muscles and muscle growth