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Refrigerated storage becomes non-negotiable to maintain its integrity, which typically lasts no more than 30 days after you've mixed it

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In the process of sperm selection (e.g., density gradient centrifugation), PDEVs can protect the integrity of sperm membranes, and OFI-EVs from cactus fruits contain plant sphingolipid components that may reduce cryo-recovery damage by stabilizing sperm membrane structure (Valentino et al., 2024), Sesame-derived extracellular vesicles (SAEVs) may also improve fertilization rates by regulating calcium ion channels to promote sperm capacitation (Zhao et al., 2025)

Each component for the ternary complex formation was co-expressed in Trichoplusia ni cells (Expression systems) with a combination of individual baculoviruses as previously described 15