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Approximately 10,000 cells were analysed per sample

It is not a drug, cosmetic, food, or supplement, and it is not intended for human consumption, therapeutic application, diagnostic use, or veterinary use

Cells were pelleted at 1000rpm, resuspended in PBS, and lysed by three freeze-thaw cycles using a dry ice ethanol bath

Painful, degenerating intervertebral discs upregulate neurite sprouting and CGRP through nociceptive factors