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We collected supernatants and processed them by centrifuging at 1500 g , 4 C, for 5 min to remove any debris

Thus, the main analytical sample included 2574 participants (2363 from NESDA baseline and 211 siblings) with data on MDD diagnostic status, overall depression severity, and depressive symptoms profiles and at least one of the investigated metabolites

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The total cost, including shipping, was $190.62