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Fourth, reverse causality remains a fundamental concernit is unclear whether dysbiosis precedes and promotes comorbidity, or whether the physiological consequences of constipation and depression (reduced motility, altered diet, medication use, stress) drive observed microbiota changes
Another concern might be the potential side effects
After 14 days of incubation, cells were stained with MTT (Sigma-Aldrich) and fixated with 2% paraformaldehyde (PFA)
However, this correlation is reliant on accurate phenotyping
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