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The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)

22 The relationship between NO and phenomena related to the spinal transmission of nociceptive inputs has been generally studied using the effects of NOS inhibitors (NOSIs) on nociception-related electrophysiological responses of dorsal horn cells after formalin

Ligand Ligand refers to the molecule attached or immobilized to the matrix, which reversibly binds to a specific target molecule

W., Schierbeek, H., Vermes, A., Huijmans, J

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