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After incubating overnight, the medium is discarded, and the cells are added with tissue culture medium, 10% FBS, containing ST2825 at concentrations ranging from 0.1 to 10 M and DMSO at 0.1% final concentration

According to the The National Center for Biotechnology Information (NCBI), intravenous administration of vitamin C has been shown to decrease oxidative stress and, in some instances, improve physiological function in adult humans

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Emamian F, Khazaie H, Okun ML, Tahmasian M, Sepehry AA

For participants studied at the NIH, the isotopes were prepared and tested for sterility and pyrogenicity by the Pharmaceutical Development Service at the NIH Clinical Center and Pine Pharmaceuticals LLC (USA) and stored for up to 6 months prior to use