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Mice were pretreated 2 hours prior to glucose with either PBS or a GIPR antagonist, 1 hour prior to glucose with either PBS or acyl-GIP (3 or 10 nmol/kg dose)

Semaglutide activates one receptor: GLP-1

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Due to semaglutide's long half-life, discontinuation at least 2 months before planned pregnancy should be considered

Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)