Taking the right steps can help you feel your best while pursuing your health goals
With ongoing provider oversight and support, patients can move forward with clarity and confidence, knowing their care is designed to work with their body, not against it
Blots were blocked in either 5% (w/v) nonfat milk (Lab Scientific, M-0841) or 1% (w/v) BSA in 1 Tris-buffered saline with Tween (TBST) (Teknova, T1688) for 3060 min at room temperature, incubated with primary antibodies in TBST for 1 h at room temperature, washed three times with TBST for 5 min each, incubated with fluorophore-conjugated secondary antibodies for 30 min at room temperature, washed three times with TBST and imaged on an Odyssey CLx imager (LI-COR) or ChemiDoc XRS+ imager with Clarity western enhanced chemiluminescence blotting substrates (Bio-Rad)
Use cold compresses
doi: 10.3389/fphar.2025.1695992 Received 31 August 2025 Revised 26 October 2025 Accepted 27 October 2025 Published 10 November 2025 Volume 16 - 2025 Edited by Galina Sudina, Lomonosov Moscow State University, Russia Reviewed by Sarah Farid, Ain Shams University, Egypt Mustafa Can Guler, Atatrk University, Trkiye Updates Copyright 2025 Lin, Alhaskawi, Chen and Moqbel