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Sample size calculation The sample size calculation was based on previous research examining the association between levels of glutathione and motivation (17), and the performance of the relevant population in the MIFT (6, 16)

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Freshly made solutions of equal volume 5,5-dithio-bis(2-nitrobenzoic acid) and glutathione reductase with concentrations of 0.33 g l 1 and 1.67 units per ml, respectively, were mixed with lysate to a final volume of 140 l

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Vitamin C serves as an essential cofactor for collagen production and can be obtained through diet or supplementation