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Exclusion criteria comprised other autoimmune diseases, CVDs, diabetes mellitus, other endocrine disorders, acute or chronic pancreatic disorders, malignancy, pregnancy, breastfeeding, or antihyperlipidemic drug usage
Myoblasts were starved for 24 h in Dulbeccos Modified Eagles Medium (DMEM, Thermo Fisher Scientific), then they were treated with one of the following conditions for 72 h: DMEM, DMEM with 10% FBS, DMEM with 1 ng mL 1 oIGF-1, or DMEM with 10 ng mL 1 oIGF-1
Tissues were incubated with primary antibodies followed by secondary antibodies overnight on a rotating platform at 4 C
UA, uric acid
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