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Other (less serious) concerns are if EGF can properly penetrate the skin (as it is a medium-sized, polar molecule, so a special delivery system is probably needed), if it can affect collagen synthesis (or just works on the surface plumping up only the upmost layers of the skin) and if it has beneficial effects at all when used in isolation versus when used in a "conditioned media" that contains lots of growth factors resembling the synergistic balance found in the skin

Herbal medicines are utilized in mixtures or singular forms for their therapeutic properties, and are often derived from bioactive compounds with medicinal potential like alkaloids, flavonoids, phenolic acids, terpenoids, and saponins [9]