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Image analysis The background was determined by manually picking regions without cells or with untransfected cells

Bacterial cells were harvested by carefully removing the supernatant by centrifugation (1,000 g , 10 min, 4 C) and the resulting pellet was resuspended with 50 l of B-PER reagent (supplemented with 1 mM DTT and 1 mM PMSF)

Additionally, SF administered at 28.2 mol/kg/day for 4 months (62), 141 mol/kg/day for 80 days (63), or 141 mol/kg/day for 5 months (64) ameliorated spatial cognitive impairment, reduced A plaques, and regulated specific HDACs, resulting in diminished plaque burden in other AD mouse models (6264)

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Girardi G, Elias MM (1991) Effectiveness of N-acetylcysteine in protecting against mercuric chloride-induced nephrotoxicity