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Description

2.5.6 Bile sample preparation A 50-L aliquot of rat bile, 50 L of 50% methanol aqueous solution, 50 L of IS solution (250 ng/mL, dissolved in acetonitrile), and 1 mL of extraction solvent (ethyl acetate: isopropanol = 3:1, v/v ) were added to tubes and vortexed for 1 min

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