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Scientists designed this synthetic pentapeptide with care, crafting it from five specific amino acids (Aib-His-D-2-Nal-D-Phe-Lys-NH)

Resin containing His-tagged 3C-HRV, noncleaved laccases and cleaved tags was discarded

Because it is prohibitively expensive to test HGF in the water maze, we instead evaluated its involvement in cognition by employing the HGF antagonist Hinge to block the actions of Dihexa

Neurite outgrowth was visualized and quantified using Phalloidin staining, which specifically binds to actin filaments

The improvements could be due to placebo effect or the invasive procedure itself (cystoscopy), and subtle side effects might have gone unnoticed in such a small sample