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All mice were from a C57BL/6 background and were group-housed and maintained in individual ventilated cages with standard bedding and enrichment in a temperature- and humidity-controlled room on a 12-h light:dark cycle (lights on 7:00) with AL access to food (standard diet, Scientific Animal Food Engineering, or 45% HFD) and water unless otherwise stated

In an extensive review, Sikiric et al

Space them by at least two hours or use on alternating days during the adjustment period

Serum total cholesterol, TAG, LDL-cholesterol, HDL-cholesterol and fasting blood glucose levels were assessed using a sophisticated Hitachi 7600-210 automated analyser

TMA, trimethylamine