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For the three- and four-channel fluorescence images in Fig 7, 405-nm excitation with 405/488/561 triband emission filter, 488-nm excitation with 525/50 emission filter, and 561-nm excitation with 605/70 emission filter were used with a triple-band 405/488/561 dichroic mirror

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Cai LL, Ruberto RA, Ryan MJ, Eaton JK, Schreiber SL, Viswanathan VS