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(5) Protein pellets were resuspended and vortexed in 250 L fresh RIPA buffer (150 mM NaCl, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0) with 0.1 mM PMSF, following which they were incubated at room temp for 5 min

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