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Tables 1 and 2 summarize passive membrane diffusion and active transport membrane permeability, respectively, for select small molecule NNMT inhibitors for which structure activity relationships had been previously developed.[17] 1-MNA, a product inhibitor of NNMT[12, 24, 25] exhibited no passive permeability (Table 1)

Colorimetric detection of GSH based on the pFeSAN In the standard procedure, the assays were performed in 1 mL HAc-NaAc buffer (0.1 M, pH 4.0) with different concentrations of GSH (0.05, 0.5, 2.5, 5, 12.5, 25, 50, 100, 150, 200, 250, 500, or 1000 M), where the TMB and pFeSAN concentrations were 1 mM and 20 g/mL, respectively

As Cl ions can directly inhibit this enzyme (Meijer et al., 1992) as well as glucose-6-phosphatase (Pederson et al., 1998) one would expect that changes in [Cl ] i may inversely correlate with glycogen biosynthesis or glucose production

Moreover, the possibility that AITC in planta can interact with oxidized glutathione -as it was shown in vitro 77 - in addition to the reduced form also needs to be investigated

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