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This work was also supported by the project of the Czech Ministry of HealthNV19-09-00578

Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h

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Every batch is supported by a downloadable Certificate of Analysis, providing full traceability and independent verification from Liquilabs s.r.o

Ethics declarations Ethics approval and consent to participate This study protocol was approved by the Ethics Committee of Shiraz University of Medical Sciences and performed in accordance with the Ethical Standards laid down in the 1964 Declaration of Helsinki and its later amendments