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Tissue preparation for fluorescent in situ hybridization (FISH) Nine male C57Bl/6 J mice (six 8 weeks old, two 14 weeks old, one 20 weeks old, 2235 g) were anesthetized with the mixture of ketamine and xylazine (ip, ketamine 50 mg/kg, xylazine 10 mg/kg body weight) and then decapitated

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