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After three washes with 200 l prewarmed PBS, 100 l of 300 M prewarmed Hanks balanced salt solution (Gibco) was added, and luminescence was immediately measured at 37 C using a Synergy H1 reader

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2018] Walsh W, Wiggins M, Fadale P, Ehrlich M (1995) Effects of a delayed steroid injection on ligament healing using a rabbit medial collateral ligament model