The infusion takes 45-60 minutes depending on your formula
Finally, the brain slices were incubated with DAPI for 5 min at room temperature

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The top 5 proteins that were up-regulated most in expression after riboflavin deficiency were H31, RPS29, AK1C2, GSH0 and TRXR1, whereas AOFB, DLD, NQO1, NDUFS1 and DDX11 were the top 5 proteins that were down-regulated most (Table 1)