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The secondary objective was to investigate the effect of duloxetine on serum BDNF in FM patients and assess the direction of BDNF changes relation to clinical parameters alterations

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Biochemical analyses were performed on maternal serum, fetal brains, and adult prefrontal cortex, measuring IL-6 and IL-17a (ELISA), parvalbumin-positive interneurons (immunofluorescence), and GABA/glutamate levels (HPLC)

In many cases, clinicians instead monitor inflammatory markers, metabolic labs, symptom patterns, and overall clinical trajectory to determine whether glutathione, its precursors, or both are appropriate

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