[DOI] [PubMed] [Google Scholar] 44.Kazamel M., Stino A.M., Smith A.G
Catalase (CAT, EC 1.11.1.6) Catalase (CAT) is a 240 kilodalton (kDa) tetrameric protein with four similar subunits and is encoded by ctt1 gene mapping to chromosome 11.33 Each polypeptide subunit is 60 kDa in weight and contains a single ferriprotoporphyrin.34 CAT is a common antioxidant enzyme present almost in all living tissues that utilize oxygen
Single-cell suspensions were then loaded onto microfluidic devices, and single-cell RNA sequencing (scRNA-seq) libraries were constructed according to the Singleron GEXSCOPE protocol via the GEXSCOPE Single-Cell RNA Library Kit (Singleron), which included cell disruption, mRNA trapping, cell labeling (with barcodes) and mRNA labeling (with unique molecular identifier (UMI)), reverse transcription of mRNA into complementary DNA (cDNA) and amplification, cDNA fragmentation, and finally cDNA fragment cloning
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