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It is most highly concentrated in the liver, where it is essential for both Phase I and Phase II detoxification pathways

The study found that 50 ng/mL TNF- caused apoptosis in the human colon epithelial cell line (NCM 460 cells) in vitro , but rhIGF-1 stimulation reduced the proportion of early apoptotic cells, as per Annexin V and 7-AAD staining flow cytometry

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