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Based on such evolving biology, the 3Ts protocol integrates all available therapeutic tools, pharmacologic oxygenation (tadalafil), intralesional biologic regeneration (tunneling), and mechanical remodeling (traction) to promote true tissue restoration rather than simple deformity correction (Table 1)

These encapsulation methods can increase the survival rate of engineered live bacteria in the gastrointestinal tract, allowing them to safely reach the intestine and stably colonize

GHK could form the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu 2+ (GHK-Cu) by the good affinity for Cu(II), which proved to exhibit good antioxidant and anti-inflammatory capabilities (Pickart, 2008)

It penetrates the outer skin layers and acts locally on skin cells, fibroblasts, and the extracellular matrix