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Ligand-mediated intracellular calcium mobilisation was determined using a FlexStation (Molecular Devices, USA), with excitation wavelength at 485 nm and emission wavelength at 525 nm every 1.5 sec for 2 minutes, including a 15-second baseline with addition of agonists at increasing concentrations
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The timeline for peptide results depends on the compound, dose, and protocol, not the reconstitution volume