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The membrane was blocked for 2 h with 5% nonfat dry milk in TBS-Tween (TBS-T: 50 mM Tris-HCl, 150 mm NaCl, and 0.1% Tween 20 (pH 7.4)), and then incubated overnight at 4 C with 1.78 g/ml GLP1R-specific antibody (MAb 3F52) 24 in TBS-T

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Molecular structure of DPP-4 Initially described by Hopsu-Havu & Glenner in 1966 [25], DPP-4 is a dimeric 240-kDa glycoprotein composed of two 120-kDa subunits and encoded by a gene located in chromosome 2q24 [25]

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