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For cell samples, BA and hepatocytes were lysed in lysis buffer (10 mM Tris pH 7.5, 5 mM EDTA, 50 mM HCl, 30 M sodium pyrophosphate, 50 mM NaF, 100 mM sodium o-vanadate, 1% Triton X-100, 1 mM PMSF and 10 g/mL protease inhibitors), pH 7.47.6

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doi: 10.1016/j.tem.2020.02.006

This practice serves multiple purposes