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All cell lines were treated with a fixed dose of MTX (25 nM) for 24 hours, as this dose was representative of MTXs average IC50 at 96 hours across all cell lines (ie, 18.4 nM)

As a result, future research should emphasize interdisciplinary collaboration, foster communication between clinical and experimental research, and ensure that the insights generated by AI and omics technologies are both verifiable and applicable in clinical settings

Significant increases warrant urological evaluation, though modest rises often occur with testosterone elevation

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