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The DIAs in the Astral analyser were performed in centroid mode for a mass range of 380980 m/z with a window width of 2 Da (without overlap), a maximum injection time of 3 ms and a normalized AGC target of 500% after fragmentation using higher-energy collisional dissociation (25% normalized collision energy)

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TT4RI = FT4 (pmol/L) TSH (mIU/L) [13]

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