Physico-chemical characterization of nanocarriers The average size, polydispersity index (PDI) and surface charge (zeta potential) of nanocarriers were measured by dynamic light scattering (Malvern Zetasizer Nano ZS, equipped with a HeNe laser ( = 632.8 nm), Malvern Instruments, UK)
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In vitro assays were performed using NUNC 96-well plates (VWR International GMBH, Darmstadt, Germany) and a CLARIOstar plate reader (BMG Labtech, Ortenberg, Germany)