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Glutathione reductase and glutathione peroxidase (GSH-Px) activities were measured using GSSG/NADPH and cumene hydroperoxide as substrates respectively as described previously ( Measurement of microsomal cytochrome P450 2E1 and 3A4 enzymes Cytochrome P450-dependent monooxygenase enzymes play important roles in maintaining redox homeostasis and detoxification of various drugs
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These findings were corroborated by in vitro experiments showing similar GLP-1 receptor signalling properties of GLP-1MK-801 and GLP-1inactive MK-801 compared with lipidated analogues of GLP-1, semaglutide and liraglutide, and the parent GLP-1 analogue (Fig
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Additionally, tirzepatide acts on areas of the brain involved in appetite regulation, leading to reduced hunger and increased feelings of fullness
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